Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKALLALFMVVSIAALAACGAGNDNQSKDNAKDGDLWASIKKKGVLTVGTEGTYEPFTYHDKDTDKLTGYDVEVITEVAKRLGLKVDFKETQWDSMFAGLNSKRFDVVANQVGKTD-REDKYDFSDKYTTSRAVVVTKKDNNDIKSEADVKGKT-SAQSLTSNYNKLATN--------AGAKVEGVEGMAQALQMIQQGRVDMTYNDKLAVLNYLKTSGNKNVKIAFETG-EPQSTYFTFRKGSGEVVDQVNKALKEMKEDGTLSKISKKWFGEDVSK 4OHN Chain:A ((8-249)) ----------------------------------GDNWSKYQSNKSITIGFDSTFVPMGFAQKD-GSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSNSYMKNEQVLVTKKSSG-ITTAKDMTGKTLGAQAGSSGYADFEANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVA-

General information: TITO was launched using: RESULT: Template: 4OHN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 -71265 -58.94 -308.50 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -58.94 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_4OHN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OHN-query.scw PDB file : Tito_Scwrl_4OHN.pdb: