Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKER-YPHLTFNIWENEPATLLELLESRQIDAAVTTTL---IKSD-TVQFKQLDDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNL-EPRIVCECHDSATLLSLVSSGFGASILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF 3FZJ Chain:A ((2-246)) LKLQTLQALICIEEVGSLRAAAQLLHLSQPALSAAIQQLEDELKAPLLVRTKRG-VSLTSFGQAFMKHARLIVTESRRAQEEIGQLRGRWEGHITFAASPAIALAALPLALASFAREFPDVTVNVRDGMYPAVSPQLRDGTLDFALTAAHKHDIDTDLEAQPLYVSDV----VIVGQRQHPMANATRLAELQECRWAFSSAPRGPGAI--IRNAFARYGLPEPKLGLVCESFLALPGVVAHSDLLTTMPRTL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 856 -129488 -151.27 -541.79 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -151.27 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_3FZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZJ-query.scw PDB file : Tito_Scwrl_3FZJ.pdb: