Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKARISIRQLFVMIIIFELGSSLLITPGSMAGRDAWIAVLLGCAIGLFLFYLYQGIYQCYPNSSPKEYMDDMLGTKLSWLFSFLYILYFAYIAARVLRDFGEMLLTFAYHDTPIIIVNALLMVVSIYAVRKGIEVLARAAELLFGAMYLLGAIGLVLIIV--SGTIDPHNLKPVLANGISPVLHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDVKKMGMIAMAISGLIVALTVAINISVLDVDLTLRSQFPLLSTIQTIKVEEFLDRLDVFFMLALIIGGFFKVSLYLYATVVGTSTLFKEKNPSQLAYPMGLGILILSITIATNFSEHLNEGLNVVPLYIHLPFQLLFPLFLFIVAVWKKKRREKSKGEEAKK 3T15 Chain:A ((68-360)) -NLDNKLDGFYIAPAFMDKLVVHITKNFLKLPNIKVPLILGIW-----------------GGKGQGKSFQCELVFRKMG-------INPIMMSAGELESGEPAKLIRQRYREAAEIIRKGNMCCLFIN----DLDNNQMVNATLMNIADNPTENARVPIIVTGNDFSTAPLIRDGR-----------MEKFYWAPTREDRIGVCTGIFRTDNVPAEDVVKIVDNFPGQSIDFFGALRARVYDDEVRKWVSGTGIEKIGDKLLNS-------------------------------------FDGPPTFEQP-----------------------------------KMTIEKLLEYGNMLVQEQENVKRVQLADK

General information: TITO was launched using: RESULT: Template: 3T15.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1007 -188492 -187.18 -722.19 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -187.18 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_3T15.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T15-query.scw PDB file : Tito_Scwrl_3T15.pdb: