Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNQMKIALLVGLAIVC-----IGLFLFYDLGNWDYTLPRRIKKVAAIVLTGGAIAFSTMIFQTITNNRILTPSILGLDSLYMLIQTGII-------FLFGSANMVIM------------NKNINFIISVLLMILFSLVLYQIMFKG-EGRNIFFLLLIGIVFGTLFSSLSSFMQMLIDPN----EFQVVQDKMFASFNNIN-----TDLLWLAFIIFLLTGVYVWR---FTKFFDVLSLGREHAVNLGIDYDKVVKQMLIVVAILVSVSTALVGPIMF-----LGLLVVNLAREFLKTYKH---SYLIAGSVFISII------ALVGGQFVVEKVFTFSTTLSVIINFAGGIYFIY--LLLKENKSW--- 4UZ1 Chain:A ((9-375)) -NEDLRLHLLLNTSVTCNDGSPAGYYLKESRGS----------RRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRT--GTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRSPVFVVQWLFDEAQLTVDNVHLPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHKTPLKGCPVHLVDSCPWPHCNPSCPTG

General information: TITO was launched using: RESULT: Template: 4UZ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1601 -310949 -194.22 -1029.63 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -194.22 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4UZ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UZ1-query.scw PDB file : Tito_Scwrl_4UZ1.pdb: