Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNFITALPIVLLLGFSFVSFMFQFEHLVYFRLALGLFSLVGLYMIYKMKTGIRYFIIYLYASWIVLAAVTAFEEPIFSSFFFGGLVMTMGYLTYMLIYLGMKQDRDANPV 2JSX Chain:A ((1-95)) MHT-----------NWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVE-----GVLAVSLVYHQQEEQGEETPRSHHHHHH

General information: TITO was launched using: RESULT: Template: 2JSX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 336 -82768 -246.33 -871.24 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -246.33 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_2JSX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JSX-query.scw PDB file : Tito_Scwrl_2JSX.pdb: