Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MTDDMTKDNINQQTLQR--GLKNRHIQLIAIGGAIGTGLFLGSGKSIHFAGPSILFAYMITGIICFLIMRSLGELLLSNLNYHSFVDFVQD---YLGDMAAF---ITGWTYWF----------CWISIAMADLTAVGLYTQYWLPGVPQWVPGLIAL----IILLIMNLATVKLFGELEFWFALIKVIAILALIVIGLV-------MIFKGFSTSSGVSSFTNLWSHGGLFPNGMHGFILSFQMVV----FAFVGIELVGLTAGETENPEKVIPKAINNIPVRVLLFYIGALLVIMSIYPWDIINP-SESPFVQVFVAVGIVGAASIINFVVLTSAASACNSAVFSTSRMVYSLAKDHNAPESMAKLTQRKVPRNALFFSAIVILIGVTL-NYIMPEGVFTLITSISTVCFIYIWGITVICHMKYRKTRPELAKTNKFK-LPLYPFTNYLILAFLAFVLVVLALAQDTRVSL----FVTPVWF-----ILLIVIYKVRKAKHQ------- 5DFM Chain:A ((1-527)) KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAA----------TGDGPDIIFWAHDRFGGYAQSGL--------LAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKSAL--MFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVG-VDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINA-ASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEI-MPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAMQQPKRNFDLYKLITDKQIDFQVADLIQDEQSSFVSVRIYGQFKCFVPKSTIQEQLDKIKNLKELAKNKIFKFLSEYNYYGYFKVQQHQFILNLENAQREASLAVDDFYFINGRIYKTNHDILILQAHHVYQMQKPTLQLLQA

General information: TITO was launched using: RESULT: Template: 5DFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2177 -339936 -156.15 -781.46 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -156.15 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_5DFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFM-query.scw PDB file : Tito_Scwrl_5DFM.pdb: