Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAWKVEPYEISKAMELIYKYLLLDKKDFSLEEFYKLTIYAIKWKLEQAQFPLYLESTKKSHQNVIPQPFKIK-------GNVLYKTVIKNSGDFEE------------ 2MK0 Chain:A ((372-459)) --EPSSQPSDCGEVIE-----------ECPIDACFL--------PKSDSARPPDCTAVGRPDCNVLPFPNNIGCPSCCPFECSPDNPMFTPSPDGSPPNCSPTMLPSPS

General information: TITO was launched using: RESULT: Template: 2MK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 166 11817 71.18 171.25 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 71.18 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_2MK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MK0-query.scw PDB file : Tito_Scwrl_2MK0.pdb: