Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------MVSIFYANRLSYSIWNTIPGKYIREEL--EQNGVTYNELIKYWDITDPSQALPKVNKDNVLLISAKHDQYIDLKDADYLWESWGRPTRYVYNCGHSGIVLC----RKKLANDTLSFIREKLV 4KEA Chain:A ((22-269)) SEQYPVLSGAEPFYAENGPVGVLLVHGFTGTPHSMRPLAEAYAKAGYTVCLPRLKGHGTHYEDMERTTFHDWVASVEEGYGWLKQRCQTIFVTGLSMGGTLTLYLAEHHPDICGIVPINAAVDIPAIAAGMTGGGELPRYLDSIGSDLKNPDVKELAYEKTPTASLLQLARLMAQTK--AKLDRIVCP-ALIFVSDENHVVPPGNADIIFQGISSTEKEIVRLRNSYHVATLDYDQPMIIERSLEFFAKHA-

General information: TITO was launched using: RESULT: Template: 4KEA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 300 -19451 -64.84 -173.67 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -64.84 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_4KEA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KEA-query.scw PDB file : Tito_Scwrl_4KEA.pdb: