Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGTEKVTALKAKHQSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPAVIATLFLVVRVLDAIFDPIMGLILDKTNTRW-GKARPYLLWVAFPFALFTFLCFTTP-HFGETGNMVYAYVTYILLGMSFSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISCATPLATAIGG-EDQAFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKKR--K-----KQG----------------------------------------IK-KTLSVLFKNKP---LLMLISSFLAFAIGFNIKLSTMVYYFTYNVNHK---------E-FVFMGTVLFFGAALISNLFIPFFSEKWGR-KQVMIITAALSLIS-YAGLHFT------PYSSIPLIFIWLF-ASGFFTTPLNTLAWGMVADCVDYAEWKTGIRADGVVISSMSFINKLGVALAGSFSAIYLGIAGYVANTDQTVASLNAIKNMNALIPGFFILLSIILIAFYPLTEKRYKHIISELEQRSAR 4TPH Chain:A ((13-453)) -------------------------SFMTVSLIELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDK----ILGTKRTML-LGAGILSVGYALMTVPTEN------TWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILV-GLGNYALMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILEYEDVARVFVYAAGVAVLGIFFHL-------ERAGLIAALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSS-W-VMIWGYASYSLGELLVSGLGLAMIAR----------------MMGAYFVASGISQYLGGVVANFASVPQDL--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1672 -257100 -153.77 -765.18 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -153.77 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: