Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------MKVKKLVVVSMLSSIAFVLMLLN---------FPFPGLPDYLKIDFSDVPAIIAILIYGPLAGIA-VEAIKNVLQYIIQGSMAGVPVGQVANFIAGTLFIL--PTAFLFKKLNSAKGLAVSLLLGTAAMTILMSILNYVLILPAYTWFLHSPALSDSALKTAVVAGILPFNMIKGIVITVVFSLIFIKLKPWIEQQRSAHIH--- 3KFV Chain:A ((3-299)) DSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTHWLAVRMGRDLREQERGIIPNQSRAEQLASLEAAQRAEDLSALTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRS----TRRLYAQAQKLRKHSSHL---FTATIPLNGTSDTWYQELKAIIREQQTRPIWTAE

General information: TITO was launched using: RESULT: Template: 3KFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 743 -27504 -37.02 -150.30 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -37.02 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3KFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KFV-query.scw PDB file : Tito_Scwrl_3KFV.pdb: