Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNKRRNRQIVVAVNGGKAVKAIFLFIVSLIVIFVLSGVLTSLRPELRPSSDSFYGIAEELPGDVFAHLLQMENHYFASDLSQTDSSFHLSRLSLKLATSINLEDPRSFLGRELPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEEREANLAEIEKQQTQSDNAQKDPPKQTTGDKKVVFIYHTHNTESYLPLLKGETDPDMARHSKANVTLVGDMFGQALESQGIGATVNKTDIQSKLNKKGLNYAR-----------SYDESRPVVKDALASNKNLQYIIDIHRDSRRKKDTTATIKGKSYARVAFVVGKKSKNFEEN-----YKIASELHKLMEKKYPGLSTGVFSKGSPGDNGVYNQDLTDRALLLEFGGVDNNLEELQRAANAAADVFSEMYWDAEKVNAASGETKKQ 4ZX5 Chain:A ((253-390)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SRLNLVAVSDFNVGAMENKGLNIFNANSLLASKKNSIDFSYARILTVVGHEYFHQYTGNRVTLRDWF--QLTLKEGLTVHRENLFSEEMTKTVT----TRLSHVDLLRSVQFLEDSSPLSHPIRPESYVSMENFY---TTTVYDKGS-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZX5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 359 15320 42.67 125.57 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 42.67 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.027

(partial model without unconserved sides chains): PDB file : Tito_4ZX5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZX5-query.scw PDB file : Tito_Scwrl_4ZX5.pdb: