Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVIFLQDVKGKGKKGEVKNVADGYAHNFLIKKGLAVEANASNISALNGQKQKEKKEAIAELEQAKSLKETLEKL-TVELSAKSGEGGRLFGSVTSKQITEQLQKDHNIKVDKRKLELPDGI-RALGYTNVPVKLHPEVQAVLKVHVKEEA 5GAG Chain:H ((1-149)) MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTA-AGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE-

General information: TITO was launched using: RESULT: Template: 5GAG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 586 -16009 -27.32 -108.90 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : -27.32 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_5GAG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAG-query.scw PDB file : Tito_Scwrl_5GAG.pdb: