Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHSFSRFFGLGEKEQEPEIAEHDTNKEEILEIPVNAIVPNRFQPRTIFSDEKIKELAMTIHTHGIIQPIVVRHTEEEGQYELIAGERRWRAVQSLEWEKIPAIIKDFSDTETASVALIENLQREELSSIEEAHAYARLLELHDLTQEALAQRLGKGQSTIANKLRLLKLPQPVQEAIMEKKITERHARALIPLKQPELQVTLLTEIIEKSLNVKQTEDRVVKMLEQGQRKPKPRRKAFSRDTRIAMNTIRQSLSMVEDSGVKLNTEEEEFEEYIQLTIRIPK 4UMK Chain:C ((35-219)) -------------------------------ELGIDEVMPNPYQPRKVFSEDSLEELAQSIKEHGLLQPVLV--VSENGRYHLIAGERRLRASKLAKMPTIKAIVVDIEQEKMREVALIENIQREDLNPLELARSYKELLESYQMTQEELSKIVKKSRAHVANIMRLLTLSSKVQNALLEEKITSGHAKVLVGL-DGEKQELILNSIIGQKLSVRQTED----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 600 -39327 -65.54 -212.58 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -65.54 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_4UMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UMK-query.scw PDB file : Tito_Scwrl_4UMK.pdb: