Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIP-YLQEQ-GFSKTELGLVLAAVSIAYGFSKFIMGMVSDRC-NPRYFLATGLFLSAIVNILFVSMPWVTS-SVTIMFIFMFINGWFQGMGWPPCGRTMAHWFSIS-ERGTKMSIWNVAHNIGGGILAPLVTLGIAM-------FVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDYPKH----------------AFKNQE-K---------------ELTTKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFS---------PEDSRWSYFLYEYAGIPGTILCGWISDRFFKSR-RAPAGVLFMAGVFIAVLVYWLNP------AGNPLVDNIALISIGFLIYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFANAIMGFVVDRFN----------WNGGFIMLISSCILAIVFLALTWNTGKRAEHV 4TPH Chain:A ((31-479)) -----------------------------------------------MQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTV----PTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGN-YALMHKSLANYGSEPDTR-PVNKKSLAIVLALAALSVVASAIILEYEDVARVFVYAAGVAVLGIFFHL-------ERAGLIAALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW---------IAAKF-ALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR---------MMGAYFVASGISQYLGG-VVANFASVPQDLVDPLQTLPVYTNLFNKLGVAAVVCTI---------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1662 -260553 -156.77 -750.87 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -156.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: