Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLQKKSVKIVLEKKQLTKVTARVGLVLDITGSMRPLYKNGTVQNVVERILAVADQFDDNGLLDVWVYDNEFSRLKPVSEKDFSGYVDREILNNDRLHKFGRNDEPPVMKDVLRKYVTEE--PSSYPAFIVFINDGGCKK----SIKPIIEASSDKPVFWQFVGIGNGNFDFLNKLDTLEGRVIDNTNFLHIEEIDRISDDELYDALLAEFPFWLKEAKEKGIVREQEPPAEKPKKKGFFSRLFSK 3N2N Chain:F ((5-159)) ---------------------YGGFDLYFILDKSGSVLHH--WNEIYYFVEQLAHKF--ISPQLRMSFIVFSTRGTTLMKLTE-DRE-QIRQGLEELQKVLPGGDTYMHEGFERASEQIYYENRQGYRTASVIIALTDGELHEDLFFYSEREANRSRDLGAIVYAVGVKDFNETQLARIADS-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3N2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 761 -83299 -109.46 -559.05 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain F : 0.64 3D Compatibility (PKB) : -109.46 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_3N2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N2N-query.scw PDB file : Tito_Scwrl_3N2N.pdb: