Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISFSESQKLFAYFSGLIAALSLFIYYVSAQQSEGALILCITFGVIAAGIWFGPIYALAVTLIVLFVLGTLMMFFQTGQTSLFPAEEGLRMLVVWGIALLLFSFISGRIHDITAELRRSMTRLQSEIKSYVAVDRVTGFDNKQRMKLELSEEIKRAERYGNSFVFLLLHMHYFKEFKSLYGEKETDRLFQYVGQQIRTSVRETDKKFRPSDERIGIVLT--HTPAEHMPAVLTKLKKQLDTYQLENGKYV--SLTFHVCYLPYRNDIQTADQFLEELENEMMMNEL 4IOB Chain:A ((1-135)) ----------------------------------------------------------------------------------------------------------------------------------------LTSLPNRAFFEGRLSRALRDANEHREQLAVLFIDSDRFKEINDRLGHAAGDTVLVNIAMRIRGQLRESDLVARLGGDEFAVLLAPLASGADALRIADNIIASMQAPIRLSDGSTVSTSLTIGIALYPEHADTPAA----------------

General information: TITO was launched using: RESULT: Template: 4IOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 -72629 -139.94 -554.42 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -139.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_4IOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IOB-query.scw PDB file : Tito_Scwrl_4IOB.pdb: