Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKLAVCLSIVLLLLGAASPISAHTVYPVNPNAQQTTKDIMNWLAHLPNRSENRVMSGAFGGYSDVTFSMT------EENRLKNATGQSPAIYGCDYGRGWLETADITDTIDYCCNSSLISYWKSGGLPQVSLHLANPAFPS--GNYKTAISNSQYKNILD--PSTVEGKRLEALLSKIADGLTQLKNQG---VTVLFRPLHEMNGEWFWWGLTGYNQKDNERISLYKELYKKIYRYMTETRGLDNLLWVYSP----DANRDFKTDFYPGSSYVDITGLDAYFTDPYAISGYDEMLSLKKPFAFAETGPSGNIGSFDYAAFINAIRQKYPQTTYFLTWDEQLSPAANQGAQSLYQNSWTLNKGEIWNGGSLTPIAE 1GVY Chain:A ((8-250)) -------------------------TVKLVDSQATMETRSLFAFM------QEQRRHSIMFGHQHETTQGLTITRTDGTQSDTFNAVGDFAAVYG------WDTLSIVAPKAEGDIVAQVKKAYARGGIITVSSHFDNPKTDTQKGVWPVGTSWDQTPAVVDSLPGGAYNPVLNGYLDQVAEWANNLKDEQGRLIPVIFRLYHANTGSWFWWGD---KQSTPEQ---YKQLFRYSVEYLRDVKGVRNFLYAYSPNNFWDVTEANYLERYPGDEWVDVLGFDTY----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1065 -66392 -62.34 -297.72 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -62.34 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1GVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVY-query.scw PDB file : Tito_Scwrl_1GVY.pdb: