Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTTVTDALYAGCEAVVKIAWLNGLWLLFTLLGGVLFGWAPSTAAMCAVIRKWLMGQKDVPIFSLFLDTYKKEFLKVNAIGLAFSALLLILSANYHYFSASTNWLSFAVTSCTLLAGLLYIIALMYVFPLYVHYQLPLRKYIPQALLFGAMRPLTTGCMLIGCGFVLYLLYTLPGLIPFYGPCLFGLVLMFFALRGFQKTEAQHHQAG--------- 1EEX Chain:B ((46-223)) --GFLTEVGEARQG---------------TQQDEVIIAVGP---AFGLAQTVNIVGIPHKSILREVIAGIEEEGIKARVIRCFKSS-----------------DVAFVAVEGNRLSGSGISIGIQSKGTTVIHQQ-GLPPLSNLELFPQAPLLTLETYRQIGKNAARYAKRESPQPVPTLND-QMARPKYQAKSAILHIKETKYVVTGKNPQELRVA

General information: TITO was launched using: RESULT: Template: 1EEX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 931 -140632 -151.05 -832.14 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : -151.05 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_1EEX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EEX-query.scw PDB file : Tito_Scwrl_1EEX.pdb: