Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEIKEAYQQCGQIVGEYAPACFKALSYLPLKQRQASWAVLSFCHTAAS----------------------------ADEKVLPAFEAKADHVYQRTNNGKQHLWKAFDHAYRTFTLESEPFREFIAAQKEDAKPYDDLDELLMYAYRTGGAAGLMLLPILTRRKQDQLKQAAVSLGLAIQLVRFLSDLGTDQQKNRI--PRQVMQQFGYTEADLQKGTVNKAFTMTWEYIAFEAEAYLEECQDALPLFPQYSQKTVKAALHLHRAVLEKIRAKQHDVFQYHFALTETEVKQILSDI 4EA1 Chain:A ((8-256)) -------FKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDIQFLNQIKEDIQSIEKYPYEYHHFQSDRRIMMALQ----HVAQHKNIAFQSFYNLIDTVYK-----DQHFTMF----ETDA-------ELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVR-------------------------

General information: TITO was launched using: RESULT: Template: 4EA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 900 -133109 -147.90 -619.11 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -147.90 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_4EA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EA1-query.scw PDB file : Tito_Scwrl_4EA1.pdb: