Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKKTFVKKAMLTTA-----AMTSAALLTFGPDAASAKTPVDNTAVQLQHQASTNEDLNTFIDILNQCIYEQDGVYYFDSEKAVELGMTKEEAQVIATLWES---TSEFFS------IVSQCVYLEDGNYKFDTEKAVELGFTEKEALALEQFFSAVSLKIHILQAAIVLQDDVYSYDK-DAALQAGA--------TPLQADVYEKLFSALS-QEQLAAIYDMIHPQA-- 1J1T Chain:A ((6-233)) STIPSSITSGSIFDLEGDNPNPLVDDSTLVFVPLEAQHITPNGNG---WRHEYKVKESLRVAMTQTYEVFEATVKVEMSDGGKTIISQHHASDTGTISKVYVSDTDESGFNDSVANNGIFDVYVRLRNTSGNEEKFALGTMTSGETFNLRVVNNYGDVEVTAFGNSFGIPVEDDSQSYFKFGNYLQSQDPYTLDKCGEAGNSNSFKNCFEDLGITESKVTMTNVTYTRETN

General information: TITO was launched using: RESULT: Template: 1J1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1146 -44566 -38.89 -222.83 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -38.89 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.010

(partial model without unconserved sides chains): PDB file : Tito_1J1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J1T-query.scw PDB file : Tito_Scwrl_1J1T.pdb: