Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRILFIGDVVGSP------------------GRDMVKEYVPKLKTKYKPHFTIINGENAAHGKGL----TEKIYHSLIQSGADAITMGNHTWDKKEIFDFIDDVPNLVRPANFPE--------G-------TPGKGITYVKANGKELAVINLQGRTFLP--------PLDDPFLKADELIAEAA-KRTPYIFIDFHAEATSEKLALGWYTDGRASAVVGTHTHVQTADNR-------------ILP---KGTAYITDVGMTGPYDGILGMDRETIIKRFKTNLPVRFTVAEGKTTLSGVVIDIDDQTKKAVKIERILINDDHMFFE 4H2G Chain:A ((26-292)) LTILHTNDVHSRLEQTSEDSSKCVDASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEG-LI---EPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMD-KLIAQKV-RGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFD-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4H2G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1156 -133531 -115.51 -651.37 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -115.51 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_4H2G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H2G-query.scw PDB file : Tito_Scwrl_4H2G.pdb: