Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDNYFASGGTQEGLLRIQQGLTKFTD----DNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVILSRESVYTANFMKYGFTPG-MGATFIVPKKLGFSLAQEILLNAGSYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVKSRIKGLYGN 5E0N Chain:X ((18-209)) -------------ALVTLNRPERMNSMAF--DVMVPLLELLGDLRHDNSVRVVILTGAGRGFSSG------------------MEVLDNVILALRRLHQPVIAAVNGAAIGGGLCLALACDVRIAAHNAYFRAAG---GLTASELGLSYLLPRAIGTSRAFEIMLTGRDVDAAEAERIGLVSRTVPDDDLLETCFEMAQRM-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5E0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 852 -143099 -167.96 -894.37 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain X : 0.72 3D Compatibility (PKB) : -167.96 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_5E0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E0N-query.scw PDB file : Tito_Scwrl_5E0N.pdb: