Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKIFIDPGHGGSDPGATG-NGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHVNSG----GGTGFESYIYPDVGAPTTTYQSTIHSEVIQAV-DFADRGKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAARAKNAGFDAIVILES 5EMI Chain:A ((4-180)) KLLVVIDPGHGGKDSGAPGLGGLLEKDVILPIGKRVAAILEQ-H-GVQAVLTRDADFFVELQGRVEIAERVNATAFVSIHANSVDNRPDVNGLEVYYYDS----GYALAEVVRNTILQNIDTIKNRGTRKARFYVLRKSSMPSILVETGYMTGREDNPRLASREYQNQMAEAIARGILKYLQR------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1003 -89308 -89.04 -522.27 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -89.04 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_5EMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EMI-query.scw PDB file : Tito_Scwrl_5EMI.pdb: