Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVYIGNGHYCYSNSTAMFLSSIGENVSPQLVEILTGVGLGAM---IEYE-K-----NLYF----SMRDPDDGINYALNIL-GFTAEEHQQASDLDDPFPLLKQQIKQNPVILGPLDMGELTYHPN----------HKNLNGSDHYVLGYQMDNENIYVQDPAGFP-FVPLSLDQFKKAWMAERIPYRKGINKYWSTAKKVVTLDNNEIYERAIDYFKRTYRKFEKVDIGLIGREAICFYADQLLNAPITADTIRHTTFFLFQLSARRANDYAMYFKDRHSHLSVLKTEQAKVFGICHSMSVNKDWKGISEKLMKLADLEDNFRLELLKVGY 3ERV Chain:A ((46-218)) ------LDRGCEVTSLAMMLQYAGITVDKMKL-ANEIKKVDFMNDGVRGNPNEGFVGNIYTFSESGYGVYHGPLFQLAKKYLPNKAVDLTGK-----SIEELYKSVKAGQPVVIITNATFAPLDEDEFTTWETNNGDVSITYNEHCVVLIGYDQESVYIRDPLKDSLDVKVPREKFEQAWVQMGS---------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ERV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 641 24563 38.32 165.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 38.32 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_3ERV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ERV-query.scw PDB file : Tito_Scwrl_3ERV.pdb: