Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENIALESSFLEYDINEPI-KIYTGHFTIEVADDFFEIL---GEVKIAFLPKARLIFEGAISGNL-SKLFEFEKAMKSNNMMINVPGFMKSEVLISGITDGSKGNKVSGILKRSILTSAETKVNRMEFTVVNFV-NDLGRRIVHGRFK-FSGRTKLKYKDWE-------------IILDKRYDYSNKKIFDRLKNSGGYLITHVGYLKRVDDKLFDTKEVEPLISGLYWLLSFSAGRHVAIPTLEGYHNEEVIW-SKY-QVPLIDGWTNNIT-WFPKQKSPSLEHLFPKVIEKQEDPFWNKVLWEVLSWYSQAHSSS-IVENKVVSVQVALETLAWVYLIVDRKSNISKSKYKYMNAAEKFREILSRFSIDLSIPKLFIDIKDNYDDGPHLFTVFRNKIVHPTRELDFDNPIDKLHVLY--LGVWYLELL-TLGILG-YE-GSYVNRLKVPIIE--GVYEFVPWKTRDN-- 4N9N Chain:A ((604-878)) -------------NLNLIDMKLFH-HYCTKVWPTITAAKVSGPEIWRDYIPELAFDYP-----FLMHALLAFSATHLSRTE----TGL--------EQYVSSHRLDALRLLREAVLEISENNTDALVASALILIMDSLANASVDNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVIT-----KDEAEKLFNQDVDAAVRGILR-----NAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYVDSMSPSAWIFHVKGAATILTAVWPLSERSKFHNIISVDLSDLGDVINPDVGTITELVCFDESIADLYPVGLDSPYLITLAYLDKLH--REKNQGDFILRVFTFPALLDKTFLALLMTGDLGAMRIMRSYYKLLRGFATEVKDKVWFLEGVTQVLPQ

General information: TITO was launched using: RESULT: Template: 4N9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1716 1708 1.00 4.32 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 1.00 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.107

(partial model without unconserved sides chains): PDB file : Tito_4N9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N9N-query.scw PDB file : Tito_Scwrl_4N9N.pdb: