Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQLLIIFAAAAAG--------LFFFEDVRDVLKLWDIRD--------MRI-IW-YGVSIAVIVILADMAV----MKWFPSHLYDDGGINKKIFSKRSIPHIIF-------LTLLI-------AFAEEMLFRGVLQTHIGLWTASLIFAALHFRYLSKWLLFIMV----------------TAISFLLGLMY--EWTGNLFVP-MTAHFIIDAVFACQIRFEHVRRDKHDEHVESREKKSPESL---------- 2PNN Chain:A ((11-258)) LYDRRSIFDAVAQSNCQELESLLPFLQRSKKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDVARKTDSLKQFVNASYTDS-----YYKGQTALHIAIERRNMTLVTLLVENGADVQAAANGDFFKKT-KGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADISARDSVGNTVLHALVEVADNTVDNTKFVTSMYNEILILGAKLHPTLKLEEITNRKGLTPLALAASSGKIGVLAYILQREIH

General information: TITO was launched using: RESULT: Template: 2PNN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 896 -67743 -75.61 -370.18 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -75.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_2PNN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PNN-query.scw PDB file : Tito_Scwrl_2PNN.pdb: