Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------MNKTDKLLESLNVFIQKAEENQYKQLGEMVPDFPGKSNIPKYV-----EEYEKGIARLLRRQHKKFLDGLKGFVSKDSEETLKALLVFFT-----------QNLFAEDDFEEEFQELTEGFL---------------QQTIEEMAEVIMDSIDPEVPFKVLSNRTINWIKDWS---KKLAEIMKLNTHEVVEDVLTNAIENGSS------IQDIELTLKDMPQFDRERARTTAITEVLAASSAAQHESYAQSPAVKKKKWRHSGGKKNNPRENHIDLDGTVIGVDEEFQIPG--SSETCMFPRDPKLST----------RERVNCHCVLS----PVVDSKILG-------LSPEEKEEIQREVLANME------ 1LK9 Chain:A ((2-425)) MTWTMKAAEEAEAVANINCSEHGRAFLDGIISEGSPKCECNTCYTGPDCSEKIQGCSADVASGDGLFLEEYWKQHKEASAVLVSPWHRMSYFFNPVSNFISFELEKTIKELHEVVGNAAAKDRYIVFGVGVTQLIHGLVISLSPNMTATPDAPESKVVAHAPFYPVFREQTKYFDKKGYVWAGNAANYVNVSNPEQYIEMVTSPNNPEGLLRHAVIKGCKSIYDMVYYWPHYTPIKYKADEDILLFTMSKFTGHSGSRFGWALIKDESVYNNLLNYMTKNTEGTPRETQLRSLKVLKEVVAMVKTQKGTMRDLNTFGFKKLRERWVNIT---ALLDQSDRFSYQELPQSEYCNYFRRMRPPSPSYAWVKCEWEEDKDCYQTFQNGRINTQNGVGFEASSRYVRLSLIKTQDDFDQLMYYLKDMVKAK

General information: TITO was launched using: RESULT: Template: 1LK9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1384 81689 59.02 270.49 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 59.02 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_1LK9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LK9-query.scw PDB file : Tito_Scwrl_1LK9.pdb: