Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MMTGTLGTLVPIILMFAVLYFLLIRPQQKQQKAVRQMQEELKK--------GDSVVTIGGLHGTVDSI----------DESKVVIKTGDNTRLTFDRRAIREVSAAE----- 2LEZ Chain:A ((17-136)) GAGTSAAMRQATSPKTILEYIINFFTCGGIRRRNETQYQELIETMAETLKSTMPDRGAPLPENIILDDMDGCRVEFNLPGENNEAGQVIVRVSKGDHSETREIPLASFEKICRALLFRCE

General information: TITO was launched using: RESULT: Template: 2LEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 -583 -2.93 -6.54 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -2.93 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_2LEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LEZ-query.scw PDB file : Tito_Scwrl_2LEZ.pdb: