Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKKQQYVTIKGTKNGLTLHLDDACSFDELLDGLQNMLSIEQYTDGKGQKISVHVKLGNRFLYKEQEEQLTELIASKKDLFVHSIDSEVITKKEAQQIREEAEIISVSKIVRSGQVLQVKGDLLLIGDVNPGGTVRAGGNIFVLGSLKGIAHAGFNGNNQAVIAASEMLPTQLRINHVLNRSPDHIQKGNEMECAYLDTDGNMVIERLQHLAHLRPDLTRLEGGM 5AJ8 Chain:A ((182-220)) -----------------------------------------------------------------------------------------------------------------------------------------CADCAIYCLPIAGSAF--LSNCTNCRVYV---ACHQLRLKGCTN---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5AJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 116 -21818 -188.09 -559.44 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -188.09 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_5AJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJ8-query.scw PDB file : Tito_Scwrl_5AJ8.pdb: