Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLNMIRRLLMTCLFLLAFGTTFLSVSGIEAKDLSKWVQEHQEKHLKHAGLRLKALQQKQTQTTSAAEDKTKPLEEAFDWDEYPVQRVTATGYTAGAESTGKNPGDPLYGLTYSGVKVKRDLYSTVAADPSVFPIGTILFIPN--------------YGLGVVADTGSAIKGNRLDLYFETVKDVYNEWGK--KTLDVYVIKKGTGKITEDELEKLNETKSLQVFRNQYKTVKE 2AE0 Chain:X ((244-337)) -------------------------------------------------------------------------------------------------------------VKGASAVPLVGR--ASVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIGPEAGHRAGWYNHYGRVWVLKTAP-----------------------------

General information: TITO was launched using: RESULT: Template: 2AE0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 279 -7980 -28.60 -102.31 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain X : 0.62 3D Compatibility (PKB) : -28.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_2AE0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AE0-query.scw PDB file : Tito_Scwrl_2AE0.pdb: