Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHQIVTTQYGKVKG----TTENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLSY--TEL--PRQ--SEDCLYVNVFAP-DTPSKNLPVMVWIHGGAFYLGAGSEPLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFNEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASR----TMTKEQAASTSAAFLQVLGINEGQ-----LDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVHSQ--ETLDAALEYLLGKPLA-EKVADLYPRSLESQIHMMTDLLF------WRPA---------VAYASAQ------SHYAPVWMYRFDWHPKK---PPYNKAFHALELPFVFGNLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPST--EAVNWPAYHEETRETLILDSEITIENDPESEKRQKLFPSKGE 1F8U Chain:A ((9-514)) ----LVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREA-PGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGQSAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCP---NDTELVACLRTRPAQVLVN--HEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDL---AAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIF----GIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPPKAPQWPPYTAGAQQYVSLD-----------------------

General information: TITO was launched using: RESULT: Template: 1F8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2758 2781 1.01 6.19 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 1.01 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1F8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F8U-query.scw PDB file : Tito_Scwrl_1F8U.pdb: