Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIE--ADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTRSYENEEGVNVYVTEVLADTVRFMDPKPREKAAD 1SE8 Chain:A ((130-231)) --NEVLVLGNVTRDPEIRYTPAGDAVLSLSIAVNENYQDRQGQRQEKVHYIDATLWRDLAENMK-ELRKGDPVMIMGRLVNEGWT------RNSTRVEATRVEAL----------

General information: TITO was launched using: RESULT: Template: 1SE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 385 -25676 -66.69 -273.15 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -66.69 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_1SE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SE8-query.scw PDB file : Tito_Scwrl_1SE8.pdb: