Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASRHTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPE-ALGTVSIEQFKKTADMLFDNG-VLTCEI-TGGEIFVHPNANEILDYVCKK--FKKVAVLTNGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGRDIVHPTKDAEQHRKFMEYEQHVIDEFKDLIPIIPYERKRAANCGAGWKSIVISPFGEVRPCALFPK-EFSLGNIFHDSYESIFNSPLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI 4M7T Chain:A ((21-239)) -----------------------------------------------------------------------------------------------------------------MDKLFSMIEVEVNSQCNRTCWYCPNSVSKRKETGEMDPALYKTLMEQLSSLDFAGRISFHFYGEPLLCKNLDLFVGMTTEYIPRARPIIYTNGDFLTEKRLQTLTELGI-QKFIVTQHAGAKHK----------FRGVYDQLAGA---DKEK-V----------------VYL--------DHSDLVLSNRGG--------------ILD----N-------I---P----ANMSCMVPSNLAVVTVLGNVLPCFEDFNQKMVMGNIGEQHISDIWHNDKFTSFRK-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4M7T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1055 -3763 -3.57 -18.00 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -3.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_4M7T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7T-query.scw PDB file : Tito_Scwrl_4M7T.pdb: