Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MYKADYKQIAATPSFQAFLKQKRAFIVPSAIFFFVFYF------SLPVLTSYFTFLNAPA-IGAVSWAW------------LFAIAQFAMTWILSTVYS--RRAAHFDKYVSALKEDLKGEQT------------ 4P0T Chain:A ((20-172)) TATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLS

General information: TITO was launched using: RESULT: Template: 4P0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 387 -33901 -87.60 -332.36 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -87.60 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_4P0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P0T-query.scw PDB file : Tito_Scwrl_4P0T.pdb: