Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYEADEIIDCSGRLVTPGLVDPHTHLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTRAASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETELKQLRVAKKLHESQPVDLVSTFMGAHAI--PPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQS--RRYLQKAAEAGFGLKIH----ADEID---------PLGGAELAGKLKA-VSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFYLGKSTYARARAMIDEGVCVSLATDFNPGSSPTENIQLI--MSIAALHLKM--------TAEEIWHAVTVNAAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVNHVHQVMKNGTIVVNREGAILG 4F0S Chain:A ((9-392)) --------IISARWIITVETDG----------EVLENHAIAIRDGKIAAIIPAADAAGLEADERLELPDHVLMPGLINLHGH---------------SAMSLLRGLADDKALMDWLTNYIWPTEGKHVHDDFVFDGSLLAMGEM---IRGGTTTINDMYFYNAAVARAGLASG----MRTFVGCSILEFPTNYASNADDYIAKGMAERSQFLGEDLLTFT--LAPHAPYTVSDDTFRKVVTLAEQEDMLIHCHIHETADEVNNSVKEHGQRPLARLQRLGLLSPRLVAAHMVHLNDAEVELAARHGLSTAHNPASNMKLA-SGISPVSKLMDAGVAVGIGTD---GAASNNKLDMLAETRLAALLAKVGTLDPTSVPAAAAIRMATLNGARALGIADKVGSVKVGKQADLIALDLAQLETAPAFDPISHV-------------------

General information: TITO was launched using: RESULT: Template: 4F0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2157 -89036 -41.28 -250.10 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -41.28 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_4F0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F0S-query.scw PDB file : Tito_Scwrl_4F0S.pdb: