Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLSQRLAGRVAVITGGGSGIGLAAGRRMRAEGATIVVGDVDVEAGGAAADELSGL----FVPTDVCDEDAVNGLFDGAAETYGRIDIAFNNAGISPPEDNLIENTELAAWQRVQDVNLKSVYLCCRAALRHMVLAGKG-SIVNTASFVAVMGSATSQISYTASKGGVLAMSRELGVQFARQ--GIRVNALCPGPVNTPLLQELFAKNPERAARRMVHVPLGRFAEPDEIAAAVAFLASDDASFITASTFLVDGGISSAYVTPL 4RF2 Chain:A ((23-271)) -----DRLKGKVAIVTGGTLGIGLAIADKFVEEGAKVVITGRHADVGEKAAKSIGGTDVIRFVQHDASDEAGWTKLFDTTEEAFGPVTTVVNNAGIAVSKS--VEDTTTEEWRKLLSVNLDGVFFGTRLGIQRMKNKGLGASIINMSSIEGFVGDPTLG-AYNASKGAVRIMSKSAALDCALKDYDVRVNTVHPGYIKTPLVDDL--EGAEEMMSQRTKTPMGHIGEPNDIAWICVYLASDESKFATGAEFVVDGGYTA------

General information: TITO was launched using: RESULT: Template: 4RF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -125129 for 2115 contacts (-59.2/contact) + 2D Compatibility (PS) -26395 + (NN) -8155 + (LL) 1580 1D Compatibility (HY) -15600 + (ID) 4450 Total energy: -178149.0 ( -84.23 by residue) QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4RF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RF2-query.scw PDB file : Tito_Scwrl_4RF2.pdb: