Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNNTTCIQPSMISSMALPIIYILLCIVGVFGNTLSQWIFLTKIGKKTSTHIYLSHLVTANLLVCSAMPFMSIYFLKGFQWEYQSAQCRVVNFLGTLSMHASMFVSLLILSWIAISRYATLMQKDSSQETTSCYEKIFYGHLLKKFRQPNFARKLCIYIWGVVLGIIIPVTVYYSVIEATEGEESLCYNRQMELGAMISQIAGLIGTTFIGFSF----LVVLTSYYSFVSHLRKIRTCTSIMEKDLTYSSVKRHLLVIQILLIVCFLPYSIFKPIFYVLHQRDNCQQLNY-LIETKNILTCLASARSSTDPIIFLLLDKTFKKTLYNLFTKSNSAHMQSYG 4EA3 Chain:A ((131-401)) --------------------LYLAVCVGGLLGNCLVMYVILRHTKMKTATNIYIFNLALADTLVLLTLPFQGTDILLGF-WPFGNALCKTVIAID----YYNMFTSTFTLTAMSVDRYVAICHP-----------------------TSSKAQAVNVAIWALASVVGVPVAIMGSA--QVEDEEIECL---VEIPTPQDYWGPVFAICIFLFSFIVPVLVISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPVQVF-----VLAQGLGVQPSSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFR---------------

General information: TITO was launched using: RESULT: Template: 4EA3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -233500 for 1935 contacts (-120.7/contact) + 2D Compatibility (PS) -25473 + (NN) -188 + (LL) 5044 1D Compatibility (HY) -23200 + (ID) 3650 Total energy: -280967.0 ( -145.20 by residue) QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_4EA3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EA3-query.scw PDB file : Tito_Scwrl_4EA3.pdb: