Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVT-KDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAAGAATTVYMEVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQ----ALVMKKSNAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL 2YLN Chain:A ((51-281)) --------------------------------------ERINNKGTVTVGTEGTYAPFTYHDKDG---KLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMAGLKAGRFDVVANQVGLTSPERQATFDKSEPYSWS-GAVLVAHND-SNIKSIADIKGVKTAQSLTSNYGEKAKAAGAQLVPVDGLA--QSLTLIEQKRADATLNDELAVLDYLKKNPNAGVKI---VWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGK-DIS------------

General information: TITO was launched using: RESULT: Template: 2YLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1213 -87422 -72.07 -386.82 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -72.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_2YLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YLN-query.scw PDB file : Tito_Scwrl_2YLN.pdb: