Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAVDKRFKSLGFNVGIPSLVFVRSLSRDCMLVVEGERVKGFAEYRYTFYKT-----RYLPDGRMTSLKVYMENQSIKR--VLHRVASFLSFLERTKQIEQKECEKVAQ 1DYM Chain:A ((66-131)) ----------------------VESCAKNCIM----EGIPDYSQYGVTTNGTSLRLQHILPDGRVPSPRVYLLDKTKRRYEMLHLTGFEFTF-----------------

General information: TITO was launched using: RESULT: Template: 1DYM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 57 -7499 -131.56 -127.10 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -131.56 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.095

(partial model without unconserved sides chains): PDB file : Tito_1DYM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DYM-query.scw PDB file : Tito_Scwrl_1DYM.pdb: