Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLLEDTKPTHLIEMGAILPDHASYEPLLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQRGGFETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSENDEMSPESLE---YACKNENIKG--------LYLIPDYHNPTTSFMSVENRRMVADIVKKYNLFVIEDASYHLL--NKNPLPALASF-APQQVIHIASLSKSLAPGLRLAYVAVPRQYKEPI---------SKALYNLNITVSPLLAELTARTIVSNQFEVLIESHREQTIRRNQLVNRYVAGYTCLGV-ETGIFRWLLLPGKMSGAEF-EELAARLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 2VGZ Chain:A ((116-425)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------TSGSQQGLCKVFEMIINPGDNVLLDEPAYSGTLQSLHPLGCNIINVASDESGIVPDSLRDILSRWKPEDAKNPQKNTPKFLYTVPNGNNPTGNSLTSERKKEIYELARKYDFLIIEDDPYYFLQFNKFRVPTFLSMDVDGRVIRADSFSKIISSGLRIGFLTGPKPLIERVILHIQVSTLHPSTFN-QLMISQLLHEWGEEGFMAH-VDRVIDFYSNQKDAILAAADKWLTGLAEWHVPAAGMFLWIKVKGINDVKELIEEKAVKMGVLMLPGNAFYVDSSAPSPYLRASFSS-ASPEQMDVAFQVLAQLIKE--

General information: TITO was launched using: RESULT: Template: 2VGZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1355 -151850 -112.07 -532.81 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -112.07 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2VGZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VGZ-query.scw PDB file : Tito_Scwrl_2VGZ.pdb: