Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKWMTVCALCFVFFLLVSCQQKDAVPDTAKKLKAPLTGLKTEQKVTERRPVAVVVNNHP----KARPQSGLSKADIVIEALAEGQITRFLAIFQSQMPETVGPVRSAREYFVTLSNGFDSIFVHHGWSPGAKKQLESGAADYMNGLDFDGSLFWRADFSKPPHNSYTSYDYIKKAAEQKGYKLKQETNPLLFQTSDAKPANESYNVRVDYGTNNVTNLVEYNYDKKAEFYTRSSDGVITTDRETGKPVAMQNIFIVEASHHIIDQDGRRDIDLESGGKGLLFQHGNVIETDWKQVNGRIVPVKDGKWLPFVPGKTWINIVPDLDAASISKGEGV 2VDC Chain:G ((22-94)) -------CSQCGVPFCQVHCPVSNNIPDWLK--------LTSEGRLEEAYEVSQATNNFPEICGRICPQDRLCEGNCVIEQSTHGAVT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2VDC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 247 -21924 -88.76 -317.73 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain G : 0.56 3D Compatibility (PKB) : -88.76 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_2VDC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VDC-query.scw PDB file : Tito_Scwrl_2VDC.pdb: