Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRARIIYNPTSGREIFKKHLAQVLQKFEQAGYETSTHATTCAGDATHAAKEAALREFDLIIAAGGDGTINEVVNGLAPLDNRPTLGVIPVGTTNDFARALGIPREDILKAADTVINGVARPIDIGQVNGQYFINIAGGGRLTELTYDVPSKLKTMLGQLAYYLKGMEMLPSLRPTEVEIEYDGKLFQGEIMLFLVTLTNSVGGFEKLAPDSSLNDGMFDLMILKKANLAEFIRVATMALRGEHINDQHIIYT--KANRVKVNVSEKMQLNLDGEYGGMLPGEFVNLYRHIHVVMPKEKAEQLDD 4WER Chain:A ((5-300)) -KKAVLIVNPSSGGEKAKEFETLAEEKLKQLFDEVVVKQTEKGGDAEQFAREAAESHFDSVFVMGGDGTVNEGISGLAEQAYRPKFGFFPLGTVNDLARALNLPM-DPEEAIQQLDLEKTSALDVGKINDDYFMNVVAIGTI-------------KLGKLAYFISGAKHLANAQTYPFHLSLDQKEQTIESSTVLVGLTNSIGGFETLLPEAQVDDGKLHLVYLKDQSLWDAVKAVPDLLKGVDQSTDNLVYLTFKEGTISLENQEELTTNVDGDEGAALPITLKILPKHLTVYCGEE-------

General information: TITO was launched using: RESULT: Template: 4WER.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1588 -156681 -98.67 -557.58 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -98.67 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4WER.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WER-query.scw PDB file : Tito_Scwrl_4WER.pdb: