Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDEPTMYHLFYGDEVANPGTELTFFEIPRIAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSGISKRAGRDILAFQDHEGQRLVLTADEEGKGYGLPVKQSGIPEEFSFRGLGPVELTVPYAEPTLHVLTNILGFTEISREPVEGQGTAVILES-GEGGAATEIHLIERNDLPRERQGKGSVHHVAFRVRDEEELAGWHRIISREGFSNSGIVERYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIALPPYLEHRRAEIEAKLKPIQ 3OAJ Chain:A ((5-308)) -KTMGIHHITAIVGHPQENTDFYAGVLGLRLVKQTVNFDDPGTYHLYFGNEGGKPGTIITFFPWAGARQGVIGDGQVGVTSYVVP-KGAMAFWEKRLEKFNVPYTKI-ERFGEQYVEFDDPHGLHLEIVEREEGEANTWTFGE--VTPDVAIKGFGGATLLSEQPDKTADLLENIMGLERVGKE-----GDFVRYRSAGDIGNVIDLKL---TPIGRGQMGAGTVHHIAWRANDDEDQLDWQRYIASHGYGVTPVRDRNYFNAIYFREHGEILFEIATDPPGFAHDETQETMGEKLMLPVQYEPHRTQIEQGLLPFE

General information: TITO was launched using: RESULT: Template: 3OAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1777 -120930 -68.05 -399.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -68.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3OAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OAJ-query.scw PDB file : Tito_Scwrl_3OAJ.pdb: