Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTYHPFSLTTPSTLMIQDWAQTNQNNKEVIAGFTTKNGGVSQKPFESLNTGLHVHDKDADVVKNREYIADMFNTDLQSWVFADQTHDNRVQKVTQRDRGKGAREYHTALKATDGIYTNEKNVFLALCFADCVPLFFYDPVKSLVGVAHAGWKGTVKQIGREMVKQWTEKEGSNPSDIYAVIGPSISGACYTVDDRVMDAVRALPVSADLAANQTAKAQYQLDLKELNRLILMDSGLASEQISVSGLCTESEPSLFYSHRRDQGKTGRMMSFIGMKEA 1Z9T Chain:A ((14-252)) ------------SKLIVPQWPQP----KGVAACSSTRIGGVSLPPYDSLNLGAHCGDNPDHVEENRKRLFAAGNLPSKP-VWLEQVHGKDVLKLTG-----------YASKRADASYSNTPGTVCAVMTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFAD----NPENILAWLGPAIGPRAFEVGGEVREAFMAVDAKASAAFIQHGD-KYLADIYQLARQRLANVGV--EQIFGGDRCTYTENETFFSYRRDK-TTGRMASFI-----

General information: TITO was launched using: RESULT: Template: 1Z9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1451 -140756 -97.01 -593.91 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -97.01 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_1Z9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z9T-query.scw PDB file : Tito_Scwrl_1Z9T.pdb: