Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTKADNRHLYLKVIERIKEDIKNGIYTEKEKLPSEFELSKKLGVSRATLREALRILEEEHVIIRRHGVGTFVHSKPLFLSGIEQLNSVTKMIEQANMTPGTIFLSSQVLMPSEDDTKRFHLAEGQELFYLERVRTADGQPIVYCIDKIPMNILPN-SFSHQQESMFDLLEKNSGSVISYAVTDIEPIGYHDTISPVLECDPETALLLLKQTHYDQHDKPVLYSLNYFRADKFRFHVLRKRF 4U0W Chain:A ((14-238)) ---------IYYQIMEQLKTQIKNGELQPDMPLPSEREYAEQFGISRMTVRQALSNLVNEGLLYRLKGRGTFV-SKPKMEQALQGLTSFTEDMKSRGMTPGSRLIDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQSSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFEHAKSVYRGDRYTF-------

General information: TITO was launched using: RESULT: Template: 4U0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 993 -92624 -93.28 -413.50 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -93.28 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_4U0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U0W-query.scw PDB file : Tito_Scwrl_4U0W.pdb: