Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVGDKIHNTNEQITALEKKKYQIETTLLEKQRDLLKLETQQNKAKLELLF------------ELSEVLTQLEGEEWVSATIALRIIKRNKRKYLDLFDLNDDKAYVNKDKFKFLHDEFFELKQQLNDI 3RJO Chain:A ((259-343)) -------------------SKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILT-LIGRNPVGYPLAWQFLRKNWNKLVQKFELGSSS-------------------------

General information: TITO was launched using: RESULT: Template: 3RJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 214 -5993 -28.00 -83.24 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -28.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_3RJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RJO-query.scw PDB file : Tito_Scwrl_3RJO.pdb: