Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVQEALNLLKENGYKYTNKREDMLQLFADSDRYLTAKNVLSALNDDYPGLSFDTIYRNLSLYEELGILETTELS-GEKLFRFKCSFTHHHHHFICLACGKTKEIESCPMD---KLCDDLDGYQVSGHKFEIYGTCPDCTAENQENTTA 3F8N Chain:A ((6-140)) --LKEALETLKETGVRITPQRHAILEYLVNSMAHPTADDIYKALEGKFPNMSVATVYNNLRVFRESGLVK--ELTYGDASSRFDF-VTSDHYHAICENCGKIVDFHYPGLDEVEQLAAHVTGFKVSHHRLEIYGVCQECS---------

General information: TITO was launched using: RESULT: Template: 3F8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 427 -53793 -125.98 -410.63 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -125.98 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_3F8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8N-query.scw PDB file : Tito_Scwrl_3F8N.pdb: