Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPTKQHKKETFTHFIFRVFMILVGAASAAVSIELFLIPNDFIDGGIIGVSLILDHLFMSNPFLNFAFFVVILNIPFMIFGYKY--------IGKTFLVSTFI-GIVGLAVIESSLHHVEAITTQPILATVFGGLLLGFGVGLVIR---NGGSMDGTEILGILLTKKLPFSVGEFVMFINVFIFIWAVFVFGPEQAMYSFMTYYIAMKTIDAVIQGLDETKAVIIVSEQ-YDEISDAILHRLGRGTTKLKGKGGYTDEEKDVIYAVVTRLEVTKLKSIVFEVDQNAFITIMNTHETRGGKFKNAIH 4PDD Chain:A ((67-227)) -----------------------------------------------------------TGPLGNFVPETRIVDIPFLFRDYEHARKVMDGAIGQDLLKKMQAKGLIGLAWTENGFRHMTN-SKRPILQA-------SDAAGLKVRTMENKVHMDGYKTFGLLPTP---------MAFPELFTALQQGTVDGQENPIPVILSSKFSQVQKHLSLTGHVYSPAVLILSSRVWDKLSEA---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 721 -38227 -53.02 -258.29 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -53.02 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.093

(partial model without unconserved sides chains): PDB file : Tito_4PDD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PDD-query.scw PDB file : Tito_Scwrl_4PDD.pdb: