Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVQEHKQEAPDIVRCKVITVSD--TRTDETDKSGKLMISFLEEAGHHIAAYEIVKDEKDALQRSVLAGCMDEQTDAVLLNGGTGIADRDVTIEAITPLFSKELPGFGEIFRMLSYTEDIGSAAIMSRATAGVIQH----TAVFSTPGSSGAVKLAMNKLIIPELAHVVREIRKDK 4LHB Chain:A ((28-186)) ----------PKTFKFGVITVSDKGAKGEREDKSGPLIIEELSKLGEHVY-YKIVPDDKIEVLIALFE-AIKSGADVVVTTGGTGITRRDITIESIKPLFDKEL-SFGEVFRAKSY-EEVGYATVLTRATAGIIRGQERIVVVFSLPGSVNAVKTGL-EIIKSEVFHILKHARE--

General information: TITO was launched using: RESULT: Template: 4LHB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 790 17068 21.61 111.56 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 21.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_4LHB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LHB-query.scw PDB file : Tito_Scwrl_4LHB.pdb: