Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLK------LTVFESDKWSFEAETETVAGIPAG-TDNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEG------HPDNAGASLVGGLVIGLHEDDETQMIRVPNADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTIL-VMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV 4RPF Chain:A ((2-288)) ------MVKIYAPASIGNVSVGFDVLGAAVSPIDGTLLGDCVSVTAAERFSLHNEGRFVSKLPDDPKQNIVYQCWERFCQEMGKEIP-VAMVLEKNMPIGSGLGSSACSVVAGLMAMNEFCGQPLDKVTLLGMMGELEGRVSGSIHFDNVAPCYLGGMQLILEQEGYISQDVPGFSDWLWVMAYPGIKVSTAEARAILPAQYRRQDCITHGRNLAGFIHACHTQQPDLAAKMMK-DVIAEPYRTQLLPGFAAARQAAQDIGALACGISGSGPTLFAVCNDQATAQRMAGWLQNHY----------------------------

General information: TITO was launched using: RESULT: Template: 4RPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1499 -290 -0.19 -1.06 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -0.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_4RPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RPF-query.scw PDB file : Tito_Scwrl_4RPF.pdb: